Tnfaip3 Polyclonal Antibody for Mouse(Mus musculus) | EIAab.comTnfaip3 Polyclonal Antibody for Mouse(Mus musculus) | EIAab.com

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Tnfaip3 (Gene name),

Tumor necrosis factor alpha-induced protein 3 (Protein name ), TNAP3_MOUSE from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

Tnfaip3(Tnfip3);

Protein name:

Tumor necrosis factor alpha-induced protein 3(TNF alpha-induced protein 3);

Alternative:

Zinc finger protein A20;Putative DNA-binding protein A20;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Homodimer. Interacts with NAF1, TAX1BP1 and TRAF2. Interacts with RNF11, ITCH and TAX1BP1 only after TNF stimulation; these interaction are transient and they are lost after 1 hour of stimulation with TNF.

Function:

Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.

Subcellular Location:

Cytoplasm Nucleus

Protein Attributes:

Sequence length:

775

Sequence:

50:

MAEQLLPQAL | YLSNMRKAVK | IRERTPEDIF | KPTNGIIYHF | KTMHRYTLEM |

100:

FRTCQFCPQF | REIIHKALID | RSVQASLESQ | KKLNWCREVR | KLVALKTNGD |

150:

GNCLMHAACQ | YMWGVQDTDL | VLRKALCSTL | KETDTRNFKF | RWQLESLKSQ |

200:

EFVETGLCYD | TRNWNDEWDN | LVKMASADTP | AARSGLQYNS | LEEIHIFVLS |

250:

NILRRPIIVI | SDKMLRSLES | GSNFAPLKVG | GIYLPLHWPA | QECYRYPIVL |

300:

GYDSQHFVPL | VTLKDSGPEL | RAVPLVNRDR | GRFEDLKVHF | LTDPENEMKE |

350:

KLLKEYLIVM | EIPVQGWDHG | TTHLINAAKL | DEANLPKEIN | LVDDYFELVQ |

400:

HEYKKWQENS | DQARRAAHAQ | NPLEPSTPQL | SLMDIKCETP | NCPFFMSVNT |

450:

QPLCHECSER | RQKNQSKLPK | LNSKLGPEGL | PGVGLGSSNW | SPEETAGGPH |

500:

SAPPTAPSLF | LFSETTAMKC | RSPGCPFTLN | VQHNGFCERC | HARQINASHT |

550:

ADPGKCQACL | QDVTRTFNGI | CSTCFKRTTA | EPSSSLTSSI | PASCHQRSKS |

600:

DPSQLIQSLT | PHSCHRTGNV | SPSGCLSQAA | RTPGDRAGTS | KCRKAGCMYF |

650:

GTPENKGFCT | LCFIEYRENK | QSVTASEKAG | SPAPRFQNNV | PCLGRECGTL |

700:

GSTMFEGYCQ | KCFIEAQNQR | FHEARRTEEQ | LRSSQHRDMP | RTTQVASRLK |

750:

CARASCKNIL | ACRSEELCME | CQHLSQRVGS | VAHRGEPTPE | EPPKQRCRAP |

775:

ACDHFGNAKC | NGYCNECYQF | KQMYG

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

Pfam:

String:

SMR:

UniGene:

STRING:

10090.ENSMUSP00000101167

Uniprot:

FOR

ELISA Kit for Mouse TNF alpha-induced protein 3

Cat.:

E9843h

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MSDS:

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Packing:

96T

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ELISA Kit for Mouse TNF alpha-induced protein 3

Cat.:

E9843m

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MSDS:

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Packing:

96T

CLIA Kit for Mouse TNF alpha-induced protein 3

Cat.:

U9843h

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MSDS:

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96T

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CLIA Kit for Mouse TNF alpha-induced protein 3

Cat.:

U9843m

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MSDS:

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Packing:

96T

Polyclonal Antibody for Mouse TNF alpha-induced protein 3

Cat.:

P9843Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse TNF alpha-induced protein 3

Cat.:

P9843Rb-h

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Packing:

40ug/0.2ml

Monoclonal Antibody for Mouse TNF alpha-induced protein 3

Monoclonal Antibody for Mouse TNF alpha-induced protein 3

Protein for Mouse TNF alpha-induced protein 3

Protein for Mouse TNF alpha-induced protein 3

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

s

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.2%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of TNAP3 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	98	90-104
Plasma	99	91-107

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNAP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-105%	93-102%	95-100%	92-106%
Saliva(n=3)	92-106%	91-108%	90-107%	90-110%
heparin plasma(n=5)	93-104%	94-102%	96-103%	95-101%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Lymphoid expression and regulation of A20, an inhibitor of programmed cell death."
Tewari M. , Wolf F.W. , Seldin M.F. , O'Shea K.S. , Dixit V.M. , Turka L.A.
J. Immunol.154:1699-1706(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"The zinc finger protein A20 inhibits TNF-induced NF-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-B-inhibiting protein ABIN."
Heyninck K. , De Valck D. , Vanden Berghe W. , Van Criekinge W. , Contreras R. , Fiers W. , Haegeman G. , Beyaert R.
J. Cell Biol.145:1471-1482(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION
3.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: C57BL/6J.
tissue: Bone marrow.
4.

"Functional redundancy of the zinc fingers of A20 for inhibition of NF-kappaB activation and protein-protein interactions."
Klinkenberg M. , Van Huffel S. , Heyninck K. , Beyaert R.
FEBS Lett.498:93-97(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH TNIP2; TAX1BP1; IKBKG AND TNIP1
5.

"The ubiquitin-modifying enzyme A20 is required for termination of Toll-like receptor responses."
Boone D.L. , Turer E.E. , Lee E.G. , Ahmad R.C. , Wheeler M.T. , Tsui C. , Hurley P. , Chien M. , Chai S. , Hitotsumatsu O. , McNally E. , Pickart C. , Ma A.
Nat. Immunol.5:1052-1060(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;CATALYTIC ACTIVITY;MUTAGENESIS OF ASP-100 AND CYS-103
6.

Boone D.L. , Turer E.E. , Lee E.G. , Ahmad R.C. , Wheeler M.T. , Tsui C. , Hurley P. , Chien M. , Chai S. , Hitotsumatsu O. , McNally E. , Pickart C. , Ma A.
Nat. Immunol.6:114-114(2005)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
7.

"The ubiquitin-editing enzyme A20 restricts nucleotide-binding oligomerization domain containing 2-triggered signals."
Hitotsumatsu O. , Ahmad R.C. , Tavares R. , Wang M. , Philpott D. , Turer E.E. , Lee B.L. , Shiffin N. , Advincula R. , Malynn B.A. , Werts C. , Ma A.
Immunity28:381-390(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
8.

"The ubiquitin-editing enzyme A20 requires RNF11 to downregulate NF-kappaB signalling."
Shembade N. , Parvatiyar K. , Harhaj N.S. , Harhaj E.W.
EMBO J.28:513-522(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TAX1BP1; RNF11 AND RIPK1
9.

"Inhibition of NF-kappaB signaling by A20 through disruption of ubiquitin enzyme complexes."
Shembade N. , Ma A. , Harhaj E.W.
Science327:1135-1139(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH UBE2N;MUTAGENESIS OF CYS-103
10.

"Immune responsive gene 1 (IRG1) promotes endotoxin tolerance by increasing A20 expression in macrophages through ROS."
Li Y. , Zhang P. , Wang C. , Han C. , Meng J. , Liu X. , Xu S. , Li N. , Wang Q. , Shi X. , Cao X.
J. Biol. Chem.288:16225-16234(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INDUCTION

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